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2008 Annual Meeting Abstracts
“mRNA Analysis of Inflammatory Cytokines in Normal Prostate Epithelial Cells, Benign Prostatic Hyperplasia Cells, and Human BPH Tissue Samples”
Alyssa Park*, Myra Wilkerson, Seema Gupta, Mansoor Ahmed, Daniel B. Rukstalis
Geisinger Medical Center, Danville, PA
Introduction:
The histologic finding of benign prostatic hyperplasia associated with chronic inflammation occurs in up to 80% of men, suggesting a role for inflammation in BPH. We investigated the expression levels of 84 inflammatory cytokines in a BPH cell line and in BPH patient samples, as compared to those in a normal prostate epithelial cell line.
Methods:
Total RNA was isolated from the BPH-1 cell line, the PReC cell line, and the paraffin-embedded tissue specimens from 6 BPH patients obtained from transurethral resections of the prostate. First strand cDNA synthesis was performed with real-time PCR using the SuperArray inflammatory cytokine gene array. The array contained 84 disease-focused genes and several controls. Data was analyzed according to the ΔΔCt method (Ct=threshold cycle). Expression in PReC cells was compared with expression in BPH-1 cells and human BPH samples.
Results:
The BPH-1 cells demonstrated 380-fold increased expression of monocyte chemotactic protein (MCP-1), followed by IL-8-RB (95-fold) and IL-10-RA (100-fold), when compared to PrEC cells. Sufficient RNA was obtained from 4 of the human samples, which also revealed an average of 9000-fold increased expression of MCP-1 in 2 cases, when compared to PrEC cells.
Conclusions:
The overexpression of MCP-1 in BPH cells and clinical samples suggests a link between inflammatory cytokine overexpression and BPH cell proliferation. Subsequent investigations will examine the cause of overexpression and the effect of anti-inflammatory agents on cellular proliferation.
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